Inheritance of a ciliary remnant in mitotic Nek2 KO cells and subsequent cilia reformation after mitosis. Live-cell imaging related to Fig. 10 B. Time-lapse epifluorescence microscopy was performed with RPE1 Nek2 KO cells stably expressing Arl13B-GFP (green) and γ-tubulin-mRuby2 (red). Cells were serum starved for 24 h and subsequently serum restimulated for 24 h before inspection. Cells were inspected for 24 h with a time interval of 10 min. Speed = 3 frames per second.
