Point mutation of both SxIP motifs mediating NAV1 binding to EB1 impairs its MT plus-end tracking ability in F-actin–rich regions of GCs. Related to Fig. 3 K. Time-lapse microscopy of neurons expressing EB3-mCherry, Lifeact-Tq, and either GFP-NAV1-WT (top) or GFP-NAV1-EBmut (bottom). Time interval: 1 second.