Human parental U2OS cell stably expressing PA–GFP–α-tubulin, mRFP-CLASP2γ C-term mutant (red), and H2B-GFP (green in the merged image; shown alone in white in the right panel) after CLASP1 and CLASP2 RNAi, with MTs (magenta) visualized with 20 nM SiR-tubulin. Note the presence of a chromosome bridge that results in micronuclei in telophase. Time is in hours:minutes. Related to Fig. 5.