Video 10.

Human parental U2OS cell stably expressing PA–GFP–α-tubulin, mRFP-CLASP2γ C-term mutant (red), and H2B-GFP (green in the merged image; shown alone in white in the right panel) after CLASP1 and CLASP2 RNAi, with MTs (magenta) visualized with 20 nM SiR-tubulin. Note the presence of a chromosome bridge that results in micronuclei in telophase. Time is in hours:minutes. Related to Fig. 5.


CLASP2 binding to curved microtubule tips promotes flux and stabilizes kinetochore attachments

Hugo Girão , Naoyuki Okada , Tony A. Rodrigues , Alexandra O. Silva , Ana C. Figueiredo , Zaira Garcia , Tatiana Moutinho-Santos , Ikuko Hayashi , Jorge E. Azevedo , Sandra Macedo-Ribeiro , and Helder Maiato

DOI: 10.1083/jcb.201905080
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