Time-lapse epifluorescence of a HeLa Flp-In cell expressing GFP-MPS1AA filmed through mitosis. Related to Fig. 4 A, third panel. DNA (SiR-Hoechst) is shown in red and GFP-MPS1 in cyan. Images were captured once every 2 min. 0 min = nuclear envelope breakdown. Video is displayed at 7 frames per second.