Lifetimes of individual FAs. Related to Fig. 4. Representative examples of time-lapse TIRF imaging of mCherry-zyxin (FA marker) assembly and disassembly in migrating MDA-MB-231 cells ectopically expressing APC-WT or APC-m4, as indicated. The arrows that appear during the video indicate the point of FA peak growth or maturation (maximum intensity), which was set to time = 0. Negative numbers correspond to the FA assembly phase, and positive numbers to disassembly phase. Images were acquired every 10 s. Video is shown at seven frames per second. Scale bar, 2 µm.
