Septin2 localization following Src activation. ECs expressing RapR-Src-Cerulean (magenta, top left) and Septin2-YFP fusion protein (green, top right) were imaged live following Src activation. The white arrow in the overlay (bottom left) indicates the colocalization of both Septin2 and Src to rosette structure. Time of RapR-Src activation is indicated by the “Rapamycin Added” caption. The video accompanies Fig. S1, B and C, and is displayed at 0.5 s per frame.