Video 6.

Moving cross-section of a ruffle at a single time point. Note that the activity underlying the ruffle extends at some points to the ventral surface.

Software for lattice light-sheet imaging of FRET biosensors, illustrated with a new Rap1 biosensor

Ellen C. O’Shaughnessy, Orrin J. Stone, Paul K. LaFosse, Mihai L. Azoitei, Denis Tsygankov, John M. Heddleston, Wesley R. Legant, Erika S. Wittchen, Keith Burridge, Timothy C. Elston, Eric Betzig, Teng-Leong Chew, David Adalsteinsson, and Klaus M. Hahn

DOI: 10.1083/jcb.201903019