De novo formation and fusion of ERES and ERAS with swapped fluorescence protein tags. The procedure was as in Video 1 and Video 2, except that the P. pastoris cells expressed Tip20-mCherry (ERAS) and Sec13-GFP (ERES). Frames from this video are shown in Fig. S3, A and C, and the frame rate is 5 frames per second.