mRNPs stalled at the NPCs: 1. Nuclear mRNP (marked by YFP-MS2, green) in an NXF1-depleted Cer-1/2-Mini-Dys U2OS cell is stalled at the nuclear periphery (marked by mCherry-POM121, magenta) but is not released into the cytoplasm. Top, cytoplasm; bottom, nucleus. Imaged every 1 s for 80 s, 7 fps.
