Videos of Fig. 3 A . NIH3T3 cells expressing LEM-domain proteins were transfected with siBAF siRNAs for 72 h before laser-induced nuclear rupture (red arrow) and imaged using time-lapse confocal microscopy. Cells expressing GFP-Emerin and transfected with siLaA/C siRNAs for 72 h before laser-induced nuclear rupture. The order of each video segment is as follows: LEMD2-GFP siControl (segment 1) and siBAF (segment 2); GFP-Man1 siControl (segment 3) and siBAF (segment 4); GFP-Emerin siControl (segment 5), siBAF (segment 6), and siLaA/C (segment 7); Ankle2-GFP siControl (segment 8) and siBAF (segment 9); Lap2alpha-GFP siControl (segment 10) and siBAF (segment 11); and Lap2β-GFP siControl (segment 12) and siBAF (segment 13). Frames were collected every 10 s and are displayed at 4 frames/s. Bar, 10 µm.
