GVBD of oocytes in modified ASW for clamping pHi at 6.7. Unstimulated oocytes were incubated in modified ASW to clamp pHi at 6.7. Time-lapse DIC imaging was performed every 10 s before and after 1-MA treatment. The video starts at 3 min before 1-MA treatment. “h: m: s” in frames represents hour, minutes, and seconds after 1-MA treatment. All images were acquired by focusing the microscope on the equatorial plane of GV region. When the equatorial plane was moved out of focus during the time-lapse imaging, the focus was returned on the equatorial plane (0:04:30, 0:20:40, 0:24:00, 0:27:40, 0:33:20, 0:42:00, 0:46:10, 0:57:50, 01:02:50, and 01:07:30). This video runs for 1 h 20 min. Selected images from the image sequence are shown in Fig. 5 C. Bar, 25 µm.
