MDA-MB-231 cells transfected with indicated siRNA (Scr or Vps26A KD) for 60 h were transfected with pHluorin–MT1-MMP. Cells were plated on a gelatin-coated imaging dish and videos were captured using a TIRF microscope (Fig. 2 E). Scale bar = 10 µm. 500 ms/frame.
