5-HT increases peripheral F-actin retrograde flow rates on PA soft gels (230 Pa). Video corresponds to Fig. 8 A. Cells were grown on 230-Pa sulfoSANPAH PA gels and were injected with Alexa Fluor 594–phalloidin. Images were acquired every 5 s with 2.5-min elapsed recording time. Scale bars, 5 µm.