TIRF imaging of Cdc8 and Cdc8-S125A coated F-actin after addition of GST-Pom1-WT and GST-Pom1-KD. Example image sequences of Cdc8-wt-Atto555 (cyan) decorated F-actin (Alexa488, magenta) after addition of Pom1(WT) (left) or Pom1(KD) (center) or of Cdc8-S125A-Atto555 (cyan) decorated F-actin (magenta) after addition of Pom1(WT) (right) at t = 0 s; 2 s per frame.
