Representative videos of 80-hpf beating hearts of control and itga5−/− larvae. Related to Fig. 7. A significantly higher percentage of itga5−/− larvae exhibit retrograde blood flow: 6 of 14 itga5−/− larvae (43%), compared with 1 of 7 itga5+/+ (14%) and 1 of 12 itga5+/− (8%) larvae. Video plays at 15 frames per second.