Video 4.

Amph-FL drives collapsing of vesicles into diffraction-limited tubules and fragments. GUV composition: 79.5 mol% DOPC, 5 mol% PtdIns(4,5)P₂, 15 mol% DOPS, and 0.5 mol% Oregon Green 488–DHPE. GUVs were mixed with 5 µM Amph-FL and imaged by confocal microscopy. Fluorescence signal comes from Atto 594–labeled protein. The frames are ∼400 ms apart. The video plays at five frames per second

BAR scaffolds drive membrane fission by crowding disordered domains

Wilton T. Snead, Wade F. Zeno, Grace Kago, Ryan W. Perkins, J Blair Richter, Chi Zhao, Eileen M. Lafer, and Jeanne C. Stachowiak

DOI: 10.1083/jcb.201807119