Video 1.

The amphiphysin N-BAR domain generates mobile lipid tubules from GUVs. GUV composition: 79.5 mol% DOPC, 5 mol% PtdIns(4,5)P₂, 15 mol% DOPS, and 0.5 mol% Oregon Green 488–DHPE. GUVs were mixed with 5 µM N-BAR and imaged by confocal microscopy within 10 min of mixing. Fluorescence signal comes from Atto 594–labeled protein. The frames are ∼400 ms apart. The video plays at five frames per second.

BAR scaffolds drive membrane fission by crowding disordered domains

Wilton T. Snead, Wade F. Zeno, Grace Kago, Ryan W. Perkins, J Blair Richter, Chi Zhao, Eileen M. Lafer, and Jeanne C. Stachowiak

DOI: 10.1083/jcb.201807119