KIF15 gliding filament assay with KBP from Fig. 3 . Time-lapse imaging of Alexa Fluor 594–labeled MTs gliding along surface-immobilized 1 µM His6-KIF15-N700 in the presence of 250 nM GST-KBP. Playback speed is 10 fps. Time stamps and scale bars are included in the video.