KIF18A gliding filament assay with KBP from Fig. 3 . Time-lapse imaging of Alexa Fluor 594–labeled MTs gliding along surface-immobilized 1 µM KIF18A-N480-GFP-His6 in the presence of 250 nM GST-KBP. Playback speed is 10 fps. Time stamps and scale bars are included in the video.