Example of rga3Δ cells with patch assembly–disassembly behavior. Time-lapse images of Scd2-GFP in h+ rga3Δ cells mated with h− WT. Example of an rga3Δ scd2-GFP cell (marked with asterisk in the first time point) that assembles and disassembles cortical patches before mating with a partner cell on the side. Time is in minutes. The video is shown at 10 frames/s. Bar, 3 µm. See also Fig. 5 D, bottom cell.
