Video 6.

Example of crossover severing event in a WT dark-grown hypocotyl epidermal cell that generates a growing new plus end. The cyan arrowhead marks the microtubule crossover site. The yellow > marks the new plus end. Growing microtubule ends are shown in green on the left panel, and the rest of the microtubule lattice is shown in grayscale. The middle panel shows the CLASP signal, and the right panel shows a merge of the microtubule and CLASP signal. Playback rate is 7 FPS.


CLASP stabilization of plus ends created by severing promotes microtubule creation and reorientation

Jelmer J. Lindeboom, Masayoshi Nakamura, Marco Saltini, Anneke Hibbel, Ankit Walia, Tijs Ketelaar, Anne Mie C. Emons, John C. Sedbrook, Viktor Kirik, Bela M. Mulder, and David W. Ehrhardt

DOI: 10.1083/jcb.201805047
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