Live imaging by high-resolution microscopy using a 1.49 NA objective of a cell expressing GFP::IFT52. Focusing on the distal tip reveals the transit and turnaround of IFT material during the conversion of anterograde to retrograde trains. For technical reasons, the bleaching event could not be recorded, and only the recovery phase can be presented. See Fig. 7 for a detailed legend. Video is shot in real time.
