3D view of WT trypanosomes analyzed by FIB-SEM with the stack of original data where several cells are visible with all typical organelles including the flagellum. The position of three IFT trains present in the flagellum of the cell located in the center of the field is indicated with arrowheads. The video pauses each time a new IFT train has been annotated. The same cell was used for the segmentation analysis presented in Fig. 3. The same reference numbers were used for these IFT trains (IFT2, IFT3, and IFT4). To reduce the size of the video, only a portion of the stack is shown. It does not contain the full flagellum explaining why the train labeled IFT1 is not visible.
