LLSM of macrophage ruffling. RAW 264.7 macrophages stably expressing GFP-LifeAct stimulated with LPS. Video of Fig. 1 A visualized in 3D (tilted) to visualize top and side of the cell. GFP-LifeAct fluorescence is visualized using the Orange-Hot LUT using Amira visualization software. Frames were collected every 1.38 s and are displayed at 25 frames per second (fps). Bar, 10 µm. Time stamp, min:s.
