Chromosome capture is coordinated in F-actin–dependent manner. Maximum-intensity z-projections through the nuclear region of live oocytes expressing H2B-3mEGFP to label chromosomes. Latrunculin B, or corresponding amount of DMSO, was added at NEBD onset (00:00). Video starts at 00:30 after NEBD and runs for 17 min. Time step: 3 s. Imaged area: 68 × 68 × 60 µm. Still frames from this dataset are shown in Fig. 2 C.
