F-actin and chromosome dynamics upon acute F-actin depolymerization. Maximum-intensity z-projections through the nuclear region of live oocytes expressing H2B-3mEGFP to label chromosomes and 3mCherry-UtrCH to label F-actin. Latrunculin B, or corresponding amount of DMSO, was added at NEBD onset (00:00). Video starts at 00:30 after NEBD and runs for 23 min. Time step: 5 s. Imaged area: 94 × 94 × 70.5 µm. Still frames from this dataset are shown in Fig. 2 B.
