Live cell imaging of individual events of FGF2 membrane recruitment at the plasma membrane. Time-lapse TIRF microscopy (80 ms per frame) was performed with CHO-K1 cells expressing either FGF2-GFP (left) or GFP (right) at concentrations that allow for single particle detection. Background signals were subtracted visualizing GFP particles in the direct vicinity of the plasma membrane. Bar, 6 µm.
