Live-imaging study by using Kif1bβ-EGFP–knock-in hippocampal neuron culture; related to Fig. S2. Time-lapse images of a Kif1bβGFP/GFP neuron observed with total internal reflection fluorescence microscopy with an α Plan Apochromat 100×/1.46 objective lens using an ELYRA P.1 microscope. The left side of the figure corresponds with the proximal. The data were taken in exposure time of 500 ms and saved as a video at seven frames per second. The whole video corresponds with 15 s in total. Bar, 10 µm.
