Video 7.

IMARIS view of hemocyte extravasation from pupal wing veins to sites of tissue damage. Related to Fig. 2. In vivo time-lapse imaging of hemocytes extravasating from pupal wing veins of 75-h APF pupae to sites of laser-induced wounding (asterisks) in the wing epithelium (IMARIS visualization) with time points every 2 min. Hemocytes often return to the vessel from which they came, although other hemocytes remain at the wound site for the duration of imaging. Wing epithelium actin is labeled with ubiquitous GFP-tagged Moesin; hemocyte actin is labeled by srp-Gal4–driven expression of mCherry-tagged Moesin. Scale bar represents 50 µm.


Drosophila immune cells extravasate from vessels to wounds using Tre1 GPCR and Rho signaling

Leila Thuma, Deborah Carter, Helen Weavers, and Paul Martin

DOI: 10.1083/jcb.201801013
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