Wild-type KKT4 tracks with a disassembling microtubule tip. TIRF movie of wild-type, 549SNAP-tagged KKT4 (green) tracking with a depolymerizing, Alexa Fluor 488–labeled microtubule (magenta). KKT4 concentration was 3.5 nM. To induce microtubule depolymerization, free tubulin (10 µM) was washed out from the chamber. The video corresponds to the event in Fig. 5 A. It was recorded at 5 frames per second.
