Visualization of FKBP-bead engulfment by a chimeric FRB-Draper receptor. An S2 cell, cotransfected with FRB-EXT-Draper-INT-mCherry (green) and LifeAct-EGFP (orange), was incubated with 2% DGS-Ni-NTA/0.5% atto390-PE 6.46-µm silica beads incubated with 10 nM His10-FKBP, washed, and resuspended before imaging. FKBP beads were incubated with cotransfected S2 cells in the presence of 1 µM rapamycin, allowed to settle in the imaging chamber for 5 min, and visualized at 5-min intervals by spinning-disk confocal microscopy. Engulfment of an FKBP bead from recognition to internalization is visible at left of this video. Video frame rate is 2.4 frames per second. Time in minutes.
