Single-molecule tracking of G3BP1 molecules in a SG. Tracks of TMR-labeled HaloTag-G3BP1 at an acquisition rate of 100 fps (SNAP-G3BP1 background) from the SG shown in Fig. 5 A. Overlay of raw data (gray), all localizations blurred with individual localization precision (red), and all trajectories longer than 10 frames (magenta) are shown. Number of frames, 7,000. Bar, 500 nm. For the experiment, stress had been induced by a 20-min treatment with 0.5 mM sodium arsenite before imaging.
