Expression of active GEF-H1 C53R increases Rho activity pulse amplitude and wave propagation. Time-lapse TIRF videos of the Rho activity sensor mCherry-Rhotekin-GBD with EGFP (control, left) or in combination with active GEF-H1 (EGFP-GEF-H1 C53R, right) in representative U2OS cells. Images were collected with a frame rate of 3/min. Bars, 10 µm.
