Time-lapse monitoring of early-to-late endosome conversion in KO-91 HeLa cells. Related to Fig. 2 F. HeLa cells were transfected with the early and late endosome markers 2×FYVE-mCh and GFP-RAB7. 24 h later, cells were placed in a 37°C humidified chamber supplemented with 5% CO2 for imaging. Images were taken every 30 s with a Z series of 20 sections (0.4 μm/section) for 1 h using a microscopy system (DV ELITE; DeltaVision). Videos were generated by projection of five sections. Image frames are shown at nine frames per second.
