Two filopodia tips showing differential response to mNeonGreen-VASP accumulation (examples from Fig. 6, A and D) in an RGC growth cone imaged with HILO illumination. Acquisition rate: 2 s per frame; playback speed: 12 fps. Red, membrane marker GAP-RFP; cyan, mNeonGreen-VASP. Bar, 1 µm.
