Time-lapse confocal microscopy of JHU12 cells (red) migrating on NF-derived CDM (green) or CAF-derived CDM (green). (Left) NF-derived CDM. (Right) CAF-derived CDM. Note the nondirectional cancer cell migration around the NF CDM and the directional cell migration along CAF CDM fibers. Images were taken every 7.5 min for 3 h. Display rate, 7 fps. Bar, 20 µm. Still images are shown in Fig. S2 E.