Video depicting MCF10A and DCIS.COM cells invading through fibrillar collagen I or GFR Matrigel. MCF10A and DCIS.COM cells stably expressing LifeAct-mRFP were plated in circular invasion assays and left to invade through fibrillar collagen I or GFR Matrigel for 3 d before being imaged live on an SDC microscope (100× objective, EMCCD camera) for over 9 h (one picture every 3 min).
