shDia1 cells unable to acutely deform individual collagen fibril. Time-lapse fluorescence imaging at the basal acinar surface of GFP-LifeAct (cyan), mCherry-MLC (magenta), and Alexa Fluor 647–labeled collagen (yellow) in an shDia1 acinus obtained 4 h after addition of 20 ng/ml HGF. Acini and collagen were imaged at 3-min intervals for 3 h. The z planes imaged were limited to 18 µm at the lower surface of acini and the juxtaposed collagen network. The video first shows the full acinus with LifeAct, MLC, and collagen overlaid. The video loops to show the boxed region in each channel separately. Arrowheads indicate a collagen fibril in contact with but not deformed by a cell moving within the acinus.Time is indicated in h:min. See also Fig. 5 B. Frame rate, seven frames per second; playback, 21 min/s.
