Impairment of chemically induced endocytosis using CD8–mCherry–FRB and FKBP–β2–GFP(Y-A). Example confocal live-cell imaging of rapamycin (200 nM) application to HeLa cells expressing CD8–mCherry–FRB and FKBP–β2–GFP(Y-A). Only the green channel is shown, in grayscale. See Fig. 9. Imaging rate, 0.2 frames/s. Video speed, 10 frames/s.