αCat basic patch contributes to epithelial cohesion and migration. Live imaging of part 1: mCherry-full-length-αCat-restored R2/7 cells imaged for 12 h postwounding at 20×; WT, full-length αCat (FLαCat; shown first) and charge mutant αCat (FLαCatKKR<3A; shown second) are compared. Images taken every 10 s during time course. Red arrows indicate αCat enrichments to the lamellipodial edge, which were quantified in Fig. 7 B. Part 2: Live imaging at 40× of mCherry-full-length-αCat and mutant αCatKKR<3A-expressing cells (red) coinfected with GFP-LifeAct (green). Labels and time stamps are incorporated within corresponding image frames. Note colocalization of αCat with LifeAct (yellow arrowheads) and formation of radial protrusions (white arrows) during epithelial sheet wound closure.
