EGF promotes αCat cortical recruitment independently of the cadherin–catenin complex. Live imaging of GFP-full-length-αCat-expressing A431D cells (cadherin–catenin negative) and stimulation with EGF (CN02; Cytoskeleton). Labels and time stamps are incorporated within corresponding image frames. Note that GFP–αCat is rapidly recruited to the cortex upon EGF stimulation (within 1 s), with concomitant decrease in cytoplasmic staining, and subsequent appearance of filopodia (within 5 s, red arrows).
