Time-lapse confocal microscopy. Cytoplasmic actin and tubulin rearrangements were observed on mouse neutrophil activation over a period of 47 min. Please see also Fig. S1 A. Lifeact-EGFP mouse neutrophils were primed briefly with GM-CSF and activated with C5a. Neutrophils expressed EGFP-labeled actin and were further stained for 30 min with SiR-tubulin to visualize the tubulin. Nuclear DNA was stained with Hoechst 33342. At the earlier time points, cortical F-actin was seen in a ring-like manner close to the cell membrane. GM-CSF–primed and C5a-activated neutrophils demonstrated F-actin accumulation at the leading edge of the cells and increasing MT network formation. Bar, 10 µm.
