Microtubule reorganization and centrosome motion upon serum starvation. This video shows x, y, and z projections of z stacks obtained during live imaging of micropatterned RPE1 stably expressing the centrosomal marker EGFP-centrin1 cells and transduced with MAP4-RFP to label the microtubules. In the left cell, microtubule bundling led to centrosome apical motion. In the right cell, microtubules did not form any bundles, and the centrosome remained close to the basal pole. Magenta arrows point at the bundle of microtubules, and the red arrows point at the centrosomes. Bars: (xy) 10 μm; (xz and yz) 5 μm.
