Mitotic progression of a Kif4A siRNA (1)–transfected cell showing multipolar spindle formation and erroneous chromosome segregation. Example of a Kif4A siRNA (1)–transfected cell undergoing mitosis in HeLa cells stably expressing histone H2B-RFP and GFP-tubulin. For each time point, RFP and GFP images were acquired. Left panel is DNA, and right panel is tubulin. Related to Fig. S5 (C and D). Video is displayed at five frames per second. Total time is 250 min.
