Mitotic progression of a GTSE1 siRNA–transfected cell showing a chromosome alignment defect. Example of a GTSE1 siRNA–transfected cell undergoing mitosis in HeLa cells stably expressing histone H2B-RFP and GFP-tubulin. For each time point, RFP and GFP images were acquired. Left panel is DNA, and right panel is tubulin. Related to Fig. 2 (A and B) and Fig. S1. Video is displayed at five frames per second. Total time is 305 min.
