Video 2.

Endosome dynamics in MEFs stably expressing GFP-SNX1. Representative examples of MEFs stably expressing GFP-SNX1, derived from Spastin^wt/wt (left), Spastin^wt/N384K (center), or Spastin^N384K/N384K (right) animals. Note the development of elongated GFP-SNX1 tubules in the Spastin^N384K/N384K MEFs that are slow to break from the parent endosome. Insets show a higher-magnification view of endosomal tubule fission events indicated by boxes in the main videos.

Defects in ER–endosome contacts impact lysosome function in hereditary spastic paraplegia

Rachel Allison, James R. Edgar, Guy Pearson, Tania Rizo, Timothy Newton, Sven Günther, Fiamma Berner, Jennifer Hague, James W. Connell, Jürgen Winkler, Jennifer Lippincott-Schwartz, Christian Beetz, Beate Winner, and Evan Reid

DOI: 10.1083/jcb.201609033