VopL/F do not bind to preassembled actin filaments in the presence of monomers. Related to Fig. 3 (A and B). TIRFM visualization of the assembly of the association of 549-SNAP-VopL and 549-SNAP-mDia2 with preassembled filaments in the presence of 1.5 µM Mg-ATP-actin (10% Alexa Fluor 488 actin). Initial reactions contained 1.5 µM G-actin (10% Alexa Fluor 488 actin). At t = 0 s, additional 1.5 µM actin (10% Alexa Fluor 488 actin) was flowed into the reaction chamber with 200 pM 549-SNAP-VopL or 1 nM 549-SNAP-mDia2 (white arrows). Bar, 5 µm. Time in seconds.
