HUVECs expressing mitochondrially targeted Timer protein shifts its fluorescence spectrum upon H2O2 treatment. Related to Fig. 6. Video shows the fluorescent spectrum shift of the MitoTimer protein from the unoxidized (green; 500 nm) form to oxidized form (red; 596 nm) upon addition of H2O2 (5 µM) to the imaging media. HUVECs transfected with pcDNA-MitoTimer for 24 h were imaged for 2 min (1 frame/min) before addition of H2O2 and then monitored by time-lapse confocal microscopy for 28 min (1 frame/min). The cells were imaged simultaneously in green (500 nm) and red (596 nm) channels. Bar, 10 µm. The video is displayed at 15 fps.
