Ran has distinct functions from Aurora A and Plk4 during spindle assembly of meiosis I. Time-lapse imaging of oocytes injected with mRNAs for EGFP-EB3 (inverted, black) and histone H2B-mRFP (control), dominant-negative Ran (T24N), or constitutively active Ran (Q69L). Progression through meiosis I was followed either in untreated conditions (control) or in the presence of Aurora A inhibitor 1 or centrinone. Expression of RanT24N further reduces microtubule nucleation. RanQ69L is not able to rescue the initial delay of microtubule nucleation caused by inhibition of Aurora A and Plk4; however, it is able to increase the amount of nucleated microtubules at later stages of meiosis progression. Z stacks are composed of 20 optical sections every 3 µm. Images were acquired every 15 min. NEBD corresponds to time 00:00 (hours:minutes). See also Fig. 7.
