GEF-H1−/− neutrophils do not respond to shear stress. GEF-H1−/− neutrophils were stimulated with 1 µM fMLP and allowed to settle on ICAM-1–coated surfaces for 5 min. Cells were then imaged for 5 min in the absence of shear stress and a further 5 min after exposure to 4 dynes/cm2 constant shear stress. Images were acquired every 500 ms. The display rate is 33 frames/second.
